Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced offtarget effects
Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced offtarget effects
The CRISPR-Cas9 system is a widely utilized platform for transgenic animal production in various species, although its off-target effects should be addressed. Several applications of this tool have been proposed in model animals but remain insufficient for transgenic livestock production.