Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design
Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design
Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not been well characterized. Here, we define rules that determine gRNA effectiveness for transcriptional repression in Saccharomyces cerevisiae.