Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP®R for DNA delivery
Preparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP®R for DNA delivery
Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP®R. They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising