Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenaseassociated stress responses
Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenaseassociated stress responses
Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying complex biological systems, such as tumor heterogeneity and tissue microenvironments. However, the sources of technical and biological variation in primary solid tumor tissues and patient-derived mouse xenografts for scRNA-seq are not well understood.