BART-Seq: Cost-effective massively parallelized targeted sequencing for genomics, transcriptomics, and single-cell analysis
BART-Seq: Cost-effective massively parallelized targeted sequencing for genomics, transcriptomics, and single-cell analysis
We describe a highly sensitive, quantitative, and inexpensive technique for targeted sequencing of transcript cohorts or genomic regions from thousands of bulk samples or single cells in parallel. Multiplexing is based on a simple method that produces extensive matrices of diverse DNA barcodes attached to invariant primer sets, which are all pre-selected and optimized in silico.