A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing
A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing
AsCas12a and LbCas12a nucleases are reported to be promising tools for genome engineering with protospacer adjacent motif (PAM) TTTV as the optimal. However, the C-containing PAM (CTTV, TCTV, TTCV, etc.) recognition by Cas12a might induce extra off-target edits at these non-canonical PAM sites.