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Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae in sheep
Mycoplasmal pneumonia is an important infectious disease that threatens sheep and goat production worldwide, and Mycoplasma ovipneumoniae is one of major etiological agent causing mycoplasmal pneumonia. Recombinase polymerase amplification (RPA) is an isothermal nucleic acid amplification technique, and RPA-based diagnostic assays have been described for the detection of different types of pathogens.
4/6/2023 9:00:29 PM +00:00
Lagovirus europeus GI.2 (rabbit hemorrhagic disease virus 2) infection in captive mountain hares (Lepus timidus) in Germany
Rabbit hemorrhagic disease virus (RHDV, Lagovirus europeus GI.1) induces a contagious and highly lethal hemorrhagic disease in rabbits. In 2010 a new genotype of lagovirus (GI.2), emerged in Europe, infecting wild and domestic population of rabbits and hares.
4/6/2023 9:00:23 PM +00:00
Differential expression of hemolysin genes in weakly and strongly hemolytic Brachyspira hyodysenteriae strains
Swine dysentery (SD) is a diarrheal disease in fattening pigs that is caused by the strongly hemolytic species Brachyspira (B.) hyodysenteriae, B. hampsonii and B. suanatina. As weakly hemolytic Brachyspira spp. are considered less virulent or even non-pathogenic, the hemolysin is regarded as an important factor in the pathogenesis of SD.
4/6/2023 9:00:16 PM +00:00
Culling reasons and risk factors in Estonian dairy cows
Culling is a major cost for dairy farms but also an essential part in managing herd productivity. This study aimed to identify the culling rates of Estonian dairy cows, identify the farmers’ stated reasons and risk factors for culling.
4/6/2023 9:00:09 PM +00:00
Pulmonary and systemic responses to aerosolized lysate of Staphylococcus aureus and Escherichia coli in calves
Constitutive and inducible defenses protect the respiratory tract from bacterial infection. The objective of this study was to characterize the response to an aerosolized lysate of killed bacteria, as a basis for studying the regulation and in vivo effects of these inducible innate immune responses.
4/6/2023 8:59:55 PM +00:00
Multilocus sequence typing characterizes diversity of Ureaplasma diversum strains, and intra-species variability induces different immune response profiles
Ureaplasma diversum is a pathogen found in the genital tract of cattle and associated with genital disorders such as infertility, placentitis, abortion, birth of weak calves, low sperm motility, seminal vesiculitis and epididymitis. There are few studies evaluating the genetic diversity of U. diversum strains and their influence on the immune response in cattle.
4/6/2023 8:59:48 PM +00:00
Critically appraised topic on adverse food reactions of companion animals (9): Time to flare of cutaneous signs after a dietary challenge in dogs and cats with food allergies
At this time, elimination diets followed by oral food challenges (OFCs) represent the “gold standard” for diagnosing skin-manifesting food allergies (FA) in dogs and cats. Regrettably, there is no clear consensus on how long one should wait for clinical signs to flare after an OFC before diagnosing or ruling-out a FA in a dog or a cat.
4/6/2023 8:59:41 PM +00:00
Molecular detection of porcine reproductive and respiratory syndrome virus, porcine circovirus 2 and hepatitis E virus in oral fluid compared to their detection in faeces and serum
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Porcine Circovirus Type 2 (PCV2) and Hepatitis E virus (HEV) are common and economically important viral disease causative agents detected in pig oral fluid (OF), faeces and serum at some infection stages.
4/6/2023 8:59:33 PM +00:00
Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs
Glaesserella parasuis, the causative agent of Glӓsser’s disease, is widespread in swine globally resulting in significant economic losses to the swine industry. Prevention of Glӓsser’s disease in pigs has been plagued with an inability to design broadly protective vaccines, as many bacterin based platforms generate serovar or strain specific immunity.
4/6/2023 8:59:25 PM +00:00
A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China
At present, pig industry in China is faced with the complex situation of mixed infection caused by multiple pathogens. It is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies.
4/6/2023 8:59:15 PM +00:00
Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
The aim of this study was to use RNA sequencing to investigate gene expression differences in testis tissue from Landrace and Duroc boars with high and low levels of sperm hyperactive motility. Boars with divergent phenotypes were selected based on their sperm hyperactivity values at the day of ejaculation (day 0) (contrasts (i) and (ii) for Landrace and Duroc, respectively) and on their change in hyperactivity between day 0 and after 96 h liquid storage at 18 °C (contrast (iii)).
4/6/2023 8:59:04 PM +00:00
Association among biofilm formation, virulence gene expression, and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in Northeast China
The aim of this study was to investigate the association among biofilm formation, virulence gene expression, and antibiotic resistance in P. mirabilis isolates collected from diarrhetic animals (n = 176) in northeast China between September 2014 and October 2016.
4/6/2023 8:58:56 PM +00:00
Perception of farmers about endometritis prevention and control measures for zerograzed dairy cows on smallholder farms in Rwanda
Endometritis is a prevalent uterine disease in postpartum cows. The disease reduces fertility performance and milk yield, and subsequently, productivity and profitability of dairy farms. The reduction in performance is associated with considerable economic losses on dairy farms.
4/6/2023 8:58:45 PM +00:00
Clinical signs, MRI findings and outcome in dogs with peripheral vestibular disease: A retrospective study
Vestibular dysfunction is relatively common in dogs, with a prevalence of 0.08% reported in primary veterinary care in the UK. There are several studies investigating how to differentiate between peripheral and central vestibular disease but only limited information regarding the possible underlying causes for peripheral vestibular dysfunction in dogs.
4/6/2023 8:58:37 PM +00:00
Pharmacokinetics and analgesic effects of intravenous, intramuscular or subcutaneous buprenorphine in dogs undergoing ovariohysterectomy: A randomized, prospective, masked, clinical trial
Buprenorphine is used for canine postoperative pain management. This study aimed to describe the pharmacokinetics and evaluate the analgesic efficacy of buprenorphine (Simbadol, 1.8 mg/mL) administered by different routes in dogs undergoing ovariohysterectomy.
4/6/2023 8:58:26 PM +00:00
Fatal Clostridium sordellii-mediated hemorrhagic and necrotizing gastroenteropathy in a dog: Case report
Canine hemorrhagic gastroenteritis (also canine gastrointestinal hemorrhagic syndrome) is commonly associated with Clostridium perfringens, although in some cases the etiology remains unclear. This report describes a fatal acute hemorrhagic and necrotizing gastroenteropathy in a dog associated with Clostridium sordellii, a bacterial species never before identified as the etiological agent of hemorrhagic and necrotizing gastroenteropathy in dogs.
4/6/2023 8:58:16 PM +00:00
Delayed diagnosis of fatal pneumonic canine plague: Clinical and pathologic features in two naturally infected Colorado dogs
Plague caused by Yersinia pestis is a highly infectious and potentially fatal zoonotic disease that can be spread by wild and domestic animals. In endemic areas of the northern hemisphere plague typically cycles from March to October, when flea vectors are active. Clinical forms of disease include bubonic, septicemic, and pneumonic plague. All clinical forms are uncommon in dogs and the pneumonic form is exceedingly rare.
4/6/2023 8:58:08 PM +00:00
Weight loss is linearly associated with a reduction of the insulin response to an oral glucose test in Icelandic horses
Insulin dysregulation (ID) goes along with lasting or transient hyperinsulinemia able to trigger equine laminitis, a painful and crippling foot condition. Promoting weight loss through dietary changes and physical activity is currently the main option to prevent this disease.
4/6/2023 8:58:01 PM +00:00
Schedule feasibility and workflow for additive manufacturing of titanium plates for cranioplasty in canine skull tumors
Additive manufacturing has allowed for the creation of a patient-specific custom solution that can resolve many of the limitations previously reported for canine cranioplasty. The purpose of this pilot study was to determine the schedule feasibility and workflow in manufacturing patient-specific titanium implants for canines undergoing cranioplasty immediately following craniectomy.
4/6/2023 8:57:53 PM +00:00
Arthroscopic approaches to and anatomy of the shoulder joint of cattle: A cadaver study
Arthroscopic surgery is described as a minimally invasive technique for diagnosis, exploration and treatment of joint disorders. It allows intraarticular structures to be assessed accurately, thereby improving the diagnostic capabilities, and it broadens the spectrum of surgical techniques feasible for treatment of articular pathologies in cattle.
4/6/2023 8:57:43 PM +00:00
Effect of sterilization on the canine vaginal microbiota: A pilot study
Surgical sterilization is the most effective method of contraception for dogs. It also prevents pyometra and reduces the risk of mammary tumour development. However, this procedure also has negative effects, such as urinary incontinence. Steroid hormone deprivation following gonadectomy could also affect canine vaginal mucosa conditions and the microbial community colonizing the vaginal tract.
4/6/2023 8:57:37 PM +00:00
Serological evidence for historical and present-day exposure of North American bison to Mycoplasma bovis
Mycoplasma bovis causes mastitis, otitis, pneumonia and arthritis in cattle and is a major contributor to bovine respiratory disease complex. Around the year 2000, it emerged as a significant threat to the health of North American bison. Whether healthy bison are carriers of M. bovis and when they were first exposed is not known.
4/6/2023 8:57:27 PM +00:00
Changes in renal parameters and their association with subclinical vector-borne infections in Bernese Mountain dogs
The aim of the study was to determine the prevalence of laboratory abnormalities suggestive of kidney disease in clinically healthy BMDs compared to a control population and to investigate if there is a correlation with the occurrence of antibodies to B. burgdorferi sensu lato, Ehrlichia canis, and Anaplasma (A.) spp. and with the occurrence of Dirofilaria (D.) immitis antigen.
4/6/2023 8:57:19 PM +00:00
Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected
The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red blood cell (RBC) indices.
4/6/2023 8:57:12 PM +00:00
“We have a ticking time bomb”: A qualitative exploration of the impact of canine epilepsy on dog owners living in England
Idiopathic epilepsy is a common neurological condition in dogs. Previous research has focused on clinical aspects of seizure management in dogs with idiopathic epilepsy with little attention given to the emotional and logistical challenges for their owners. The current study aimed to explore the impacts of owning a dog with idiopathic epilepsy on owner quality of life and lifestyle, using qualitative methods.
4/6/2023 8:57:02 PM +00:00
Epidemiological characterization of Mycobacterium caprae strains isolated from wildlife in the Bieszczady Mountains, on the border of Southeast Poland
The majority of animal tuberculosis (TB) cases reported in wildlife in Poland over the past 20 years have concerned the European bison inhabiting the Bieszczady Mountains in Southeast Poland: an area running along the border of Southeast Poland.
4/6/2023 8:56:55 PM +00:00
Toxoplasma gondii infection in pet cats and their owners in northeastern China:an important public health concern
Limited information about Toxoplasma gondii infection in pet cats and their owners is available in China. Methods: In this study, blood samples were randomly collected from 306 pet cats and 397 corresponding pet own‑ ers in Jilin province, northeastern China.
4/6/2023 8:56:48 PM +00:00
Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus
Epizootic haemorrhagic disease virus (EHDV) and the Palyam serogroup viruses (PALV) have led to significant economic losses associated with livestock production globally. A rapid, sensitive and specific method for the detection of EHDV and PALV is critical for virus detection, monitoring, and successful control and elimination of related diseases.
4/6/2023 8:56:38 PM +00:00
Prevalence and phylogenetic analysis of hepatitis E virus in pigs in Vietnam
Hepatitis E virus (HEV) is a zoonotic disease and has been reported around the world. The main objective of this study was to evaluate the sero-prevalence and phylogenetic analysis of HEV in Vietnam. Pig blood and fecal pooled samples were collected to assess the prevalence of HEV.
4/6/2023 8:56:29 PM +00:00
Canine transmissible venereal tumour established in immunodefcient mice reprograms the gene expression profles associated with a favourable tumour microenvironment to enable cancer malignancy
Canine transmissible venereal tumours (CTVTs) can cross the major histocompatibility complex barrier to spread among dogs. In addition to the transmissibility within canids, CTVTs are also known as a suitable model for investigating the tumour–host immunity interaction because dogs live with humans and experience the same envi‑ ronmental risk factors for tumourigenesis.
4/6/2023 8:56:19 PM +00:00