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Herpes Simplex Virus;
Type I PCR Product
5’TCAACTTCGACTGGCCCTTCTTGCTGGCCAAGCTGACG GACATTTACAAGGTCCCCCTGGAGACGGGTACGGCCGCA TGAACGGCCGGGGCGTGTTTCGCGTGTGGGACATAGGCC AGAGCCACTTCCAGAAGCGCAGCAAGATAAAGGTGAACG GCATGGTGAGCATCGACATGTACGG 3’
Type II PCR Product
5’TCAACTTCGACTGGCCCTTCGTCCTGACCAAGCTGACG GAGATCTACAAGGTCCCGCTCGAGACGGGTACGGGCGCA TGAACGGCCGGGGTGTGTTCCGCGTGTGGGACATAGGCC AGAGCCACTTCCAGAAGCGCAGCAAGATAAAGGTGAACG GCATGGTGAACATCGACATGTACGG 3’
HSV Type I infects the nervous system,
however HSV Type II infects the genital system.
In routine analysis; the discrimination between Type I and II is done by sequence detection system.
Variations of HSV Type 1 and Type 2 discriminations and probes selection :
HSV Type 1 (between 64208 and 64386 bases sequences) 5’ ATC AAC TTC GAC TGG CCC TTC *TTG CTG GCC AAG CTG
ACG GAC A * TT TAC AAG GTC CCC CTG GAC GGG TAC GGC CGC ATG AAC GGC CGG GGC GTG TTT CGC GTG TGG GAC ATA GGC CAG AGC CAC TTC CAG AAG CGC AGC AAG ATA AAG GTG AAC GGC ATG GTG AGC ATC GAC ATG TAC GG 3’
HSV Type 2 (between 64669 and 64847 bases sequences) 5’ ATC AAC TTC GAC TGG CCC TTC *GTC CTG ACC AAG CTG ACG GAG A * TC TAC AAG GTC CCG CTC GAC GGG TAC GGG CGC
ATG AAC GGC CGG GGT GTG TTC CGC GTG TGG GAC ATC GGC CAG AGC CAC TTC CAG AAG CGC AGC AAG ATA AAG GTG AAC GGC ATG GTG AAC ATC GAC ATG TAC GG 3’
Real Samples-2
Label-Free Electrochemical Hybridization Genosensor for the Detection of Hepatitis B Virus Genotype on the Development of Lamivudine Resistance
-Lamivudine is a pyrimidine nucleoside analogue that inhibits
viral replication by blocking viral reverse transcriptase
-Continuous lamivudine therapy may lead to selection of resistant strains
Anal. Chem.2005, 77,4908-4917
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