- Trang Chủ
- Ngư nghiệp
- Phương thức lây truyền của ký sinh trùng Perkinsus olseni (Lester & Davis, 1981) và thử nghiệm trị bệnh trong điều kiện thí nghiệm
Xem mẫu
- KHOA H C CÔNG NGH
PH JNG TH C LÂY TRUY N C
- KHOA H C CÔNG NGH
granosa) và hàu cLa sông (Crassostrea rivularis) 1.660 ± 593,8 bào tL/cá th3 $ %c b. trí ng9u nhiên 20
s ch b8nh $ %c thu tX nh?ng vùng nuôi không b cá th3/thùng x.p (th3 tích n #c là 10 L/thùng, kích
b8nh Perkinosis t i B c Liêu. Nhóm nghêu b8nh th #c 30 x 25 x 25 cm) C nhi8t $) là 28 - 300°C, $)
$ %c thu tX huy8n C^n GiU, thành ph. H@ Chí Minh mBn 25‰. MŠi nghi8m thbc $ %c lBp l i 3 l^n. MŠi
n i nghêu $ %c báo cáo có tP l8 nhi m Perkinsus $ n v thí nghi8m $ %c b. trí 20 cá th3 nghêu. Cá th3
cao. o kích th #c và cân kh.i l %ng tXng cá th3 nghêu ch
- KHOA H C CÔNG NGH
nhi m chéo và gây ch
- KHOA H C CÔNG NGH
B6ng 2. C Ung $) nhi m Perkinsus olseni khi $i u tr bwng Desferrioxamine
ThUi gian C Ung $) nhi m (bào tL/cá th3)
$i u tr (ngày) 10 mg/L 15 mg/L 30 mg/L .i chbng
1 1.688 ± 128 2.033 ± 51 2.031 ± 34 1.613 ± 220
2 1.858 ± 139 1.800 ± 95 1.653 ± 114 1.687 ± 150
3 1.713 ± 97 1.612 ± 91 1.840 ± 63 1.760 ± 126
4 1.379 ± 150 1.677 ± 78 1.840 ± 129 1.627 ± 282
5 1.475 ± 62 1.400 ± 65 1.550 ± 318 1.693 ± 165
6 1.519 ± 110 1.483 ± 46 1.325 ± 86 1.762 ± 199
Ghi chú: S. li8u di n gi6i giá tr trung bình ± sai s. chumn
Tuy thUi gian $i u tr trong thí nghi8m ng n (6 phát tri3n. P. marinus có th3 tránh $ %c oxy hóa gây
ngày) nh ng b #c $^u crng $ã cho th5y $ %c hi8u 6nh h Cng $
- KHOA H C CÔNG NGH
4. Elandalloussi, L. M., Leite, R. B., Rodrigues, P. 10. OIE, 2012. Manual of Diagnostic Tests for
M., Afonso, R., Nunes, P. A. & Cancela, M. L., 2005a. Aquatic Animals.
Effect of antiprotozoal drugs on the proliferation of 11. Park, K. I. & Choi, K. S., 2001. Spatial
the bivalve parasite Perkinsus olseni. Aquaculture, distribution of the protozoan parasite Perkinsus sp.
243(1-4), 9-17. found in the Manila clams, Ruditapes philippinarum,
5. Ford, S. E., Zhe, Xu & Debrosse, G., 2001. Use in Korea. Aquaculture, 203, 9-22.
of particle filtration and UV irradiation to prevent 12. Patricia, da S. M., Carolina, P. C., Jaíse, de A.
infection by Haplosporidium nelsoni (MSX) and P. B., Fernando, R. Q. & Alexandre, A. W., 2016.
Perkinsus marinus (Dermo) in hatchery-reared Epizootiology of Perkinsus sp. in Crassostrea gasar
larval and juvenile oysters. Aquaculture, 194, 37-49. oysters in polyculture with shrimps in northeastern
6. Gauthier, J. D. & Vasta, G. R., 1994. Inhibition Brazil. Brazilian Journal of Veterinary Parasitology,
of in vitro replication of the oyster parasite Perkinsus 25, 37-45.
marinus by the natural iron chelators transferrin, 13. Ray, S. M., 1952. A culture technique for the
lactoferrin, and desferrioxamine. Dev. Comp. diagnosis of infections with Dermocystidium
Immunol. 18(4), 277-286. marinum, Mackin, Owen and Collier, in oysters.
7. Goggin, C. L., Sewell, K. B. & Lester, R. J. G., Science, 116, 360-361.
1989. Cross-infection experiments with Australian 14. Ray, S. M., 1954. Biological studies of
Perkinsus species. Diseases of Aquatic Organisms, 7, Dermocystidium marinus, a fungus parasite of
55-59. oysters. Rice University, Houston, TX.
8. La Peyre, M. K., Nickens, A. D., Volety, A. K., 15. Smolowitz, R., 2013. A Review of Current
Tolley, G. S. & La Peyer, J. F., 2003. Environmental State of Knowledge Concerning Perkinsus marinus.
significance of freshets in reducing Perkinsus Effects on Crassostrea virginica (Gmelin) (the
marinus infection in eastern oysters Crassostrea Eastern Oyster). Veterinary Pathology, 50(3), 404-
virginica: potential management applications. Marine 411.
Ecology Progress Series 248, 165-176.
16. Tasumi, S. & Vasta, G. R., 2007. A galectin of
9. Mackin, J. G. & Hopkins, S. H., 1962. Studies unique domain organization from hemocytes of the
on oyster mortality in relation to natural eastern oyster (Crassostrea virginica) is a receptor
environments and to oil fields in Louisiana. for the protistan parasite Perkinsus marinus. J.
University of Texas Institute of Marine Science. Immunol, 179, 3086-3098.
TRANSMISSION OF Perkinsus olseni AND TREATMENT IN LABORATORY CONDITIONS
Pham Quoc Hung, Ngo Thi Ngoc Thuy, Nguyen Thi Hong Nhung
Summary
The protozoan parasite Perkinsus olseni is a pathogen causing mass and severe mortalities in bivalve
mollusc species in many countries in the world. In order to reduce the loss caused by this pathogen, it is
vital to determine the preventive and therapeutic measures. The current study investigated the
transmission of P. olseni among bivalve species and therapeutic efficiency of deferoxamine against
perkinsus infection. The result indicated that P. olseni can be transmitted directly between individuals of
Ben Tre clams and from infected Meretrix lyrata to cockle Anadara granosa and estuarine oyster
Crassostrea rivularis. Using deferoxamine at doses of 15 and 30 mg/L was shown to reduce intensity of P.
olseni infection.
Keywords: Ben Tre clam, Deferoxamine, Perkinsus olseni, transmission.
Ng Ui ph6n bi8n: TS. Võ
Võ Th<
Th< Drng
Ngày nh;n
nh;n bài: 26/5/2017
Ngày thông qua ph6n
ph6n bi8n:
bi8n: 29/6/2017
Ngày duy8t
duy8t $ ng: 6/7/2017
94 N«ng nghiÖp vµ ph¸t triÓn n«ng th«n - KỲ 1 - TH¸NG 3/2018
nguon tai.lieu . vn
