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- 46
Điều chỉnh pH = 7,4 bằng NaOH 1N hoặc NaOH 5N.
(f) Môi trƣờng khi nuôi
200 μl
FCS
200 μl
Pyruvic acid
200 μl
hMG
1400 μl
TCM 199
2. Trên chó
(a) Thành phần môi trƣờng
môi trường căn bản
TCM199
HEPES 25mM/l
ECS 10%
50 μg/ml
Gentamycin
NaHCO3 2,2 mg/ml
22μg/ml
Pyruvic acid
1 μg/ml
Estradiol
0,5 μg/ml
FSH
hCG 0,03 UI/ml
(b) Pha môi trƣờng căn bản TCM199
Nước cất 100ml
TCM199 980 mg
NaHCO3 220 mg
Gentamycin 5 mg
Hepes 5,97g
Trữ ở 4oC.
(c) Pha NaHCO3
TCM199 10ml
NaHCO3 0,22g
Trữ ở -20oC.
(d) Pha pyruvic acid
TCM199 10 ml
Pyruvic acid 0,022g
Trữ ở -20oC.
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(e) Pha estradiol, FSH, hCG bằng môi trường căn bản TCM199. Trữ ở -20oC.
(f) Môi trƣờng nuôi trứng chín
50 μl
Pyruvic acid
50 μl
Estradiol
50 μl
FSH
50 μl
hMG
4300 μl
TCM199
3. Môi trƣờng PBS – PVA
Nước cất 100 ml
NaCl 800 mg
KCl 20 mg
Na2HPO4.12H2O 290 mg
KH2PO4 20 mg
Polyvinyl alcohol (PVA) 100 mg
PVA được pha riêng với nước cất, gia nhiệt khoảng 70oC đến khi PVA tan hết trong
nước. Sau đó được pha chung với môi trường PBS – PVA.
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PHỤ LỤC 2. BẢNG TRONG STAGRAPHIC
Analysis of Variance for HEO.LOAIA - Type I Sums of Squares
---------------------------------------------------------------------------
-----
Source of variation Sum of Squares d.f. Mean square F-ratio Sig.
level
---------------------------------------------------------------------------
-----
MAIN EFFECTS
A:HEO.PPHAP .0591267 1 .0591267 .479 .4971
B:HEO.KTHUOC 1.8879429 2 .9439714 7.652 .0007
RESIDUAL 21.341147 173 .1233592
---------------------------------------------------------------------------
-----
TOTAL (CORRECTED) 23.288217 176
---------------------------------------------------------------------------
-----
0 missing values have been excluded.
All F-ratios are based on the residual mean square error.
Table of Least Squares Means for HEO.LOAIA
---------------------------------------------------------------------------
-----
95%
Confidence
Level Count Average Stnd. Error for mean
---------------------------------------------------------------------------
-----
GRAND MEAN 177 .4825215 .0478110 .3881326.5769104
A:HEO.PPHAP
1 84 .4960188 .0556274 .3861985.6058390
2 93 .4690242 .0536471 .3631135.5749349
B:HEO.KTHUOC
1 90 .4164220 .0370971 .3431846.4896594
2 80 .6235000 .0392682 .5459763.7010237
3 7 .4076425 .1328045 .1454584.6698265
---------------------------------------------------------------------------
---
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Analysis of Variance for HEO.LOAIB - Type I Sums of Squares
---------------------------------------------------------------------------
-----
Source of variation Sum of Squares d.f. Mean square F-ratio Sig.
level
---------------------------------------------------------------------------
-----
MAIN EFFECTS
A:HEO.PPHAP .0341841 1 .0341841 .351 .5609
B:HEO.KTHUOC 1.1146264 2 .5573132 5.716 .0039
RESIDUAL 16.868994 173 .0975086
---------------------------------------------------------------------------
-----
TOTAL (CORRECTED) 18.017805 176
---------------------------------------------------------------------------
-----
0 missing values have been excluded.
All F-ratios are based on the residual mean square error.
Table of Least Squares Means for HEO.LOAIB
---------------------------------------------------------------------------
-----
95%
Confidence
Level Count Average Stnd. Error for mean
---------------------------------------------------------------------------
-----
GRAND MEAN 177 .3639194 .0425073 .2800011.4478377
A:HEO.PPHAP
1 84 .3535265 .0494567 .2558887.4511643
2 93 .3743123 .0476960 .2801503.4684743
B:HEO.KTHUOC
1 90 .4377429 .0329819 .3726297.5028560
2 80 .2755000 .0349121 .2065761.3444239
3 7 .3785153 .1180724 .1454155.6116151
---------------------------------------------------------------------------
---
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Analysis of Variance for HEO.loaiC - Type I Sums of Squares
---------------------------------------------------------------------------
-----
Source of variation Sum of Squares d.f. Mean square F-ratio Sig.
level
---------------------------------------------------------------------------
-----
MAIN EFFECTS
A:HEO.PPHAP .0099814 1 .0099814 .146 .7066
B:HEO.KTHUOC .2376642 2 .1188321 1.742 .1782
RESIDUAL 11.800240 173 .0682095
---------------------------------------------------------------------------
-----
TOTAL (CORRECTED) 12.047886 176
---------------------------------------------------------------------------
-----
0 missing values have been excluded.
All F-ratios are based on the residual mean square error.
Table of Least Squares Means for HEO.loaiC
---------------------------------------------------------------------------
-----
95%
Confidence
Level Count Average Stnd. Error for mean
---------------------------------------------------------------------------
-----
GRAND MEAN 177 .1619216 .0355520 .0917345.2321087
A:HEO.PPHAP
1 84 .1711807 .0413643 .0895189.2528425
2 93 .1526625 .0398918 .0739078.2314172
B:HEO.KTHUOC
1 90 .1691564 .0275852 .1146974.2236153
2 80 .1010000 .0291996 .0433538.1586462
3 7 .2156084 .0987528 .0206497.4105672
---------------------------------------------------------------------------
---
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Analysis of Variance for CHO5.A - Type I Sums of Squares
---------------------------------------------------------------------------
-----
Source of variation Sum of Squares d.f. Mean square F-ratio Sig.
level
---------------------------------------------------------------------------
-----
MAIN EFFECTS
A:CHO5.buongtrung .0001369 1 .0001369 .002 .9652
B:CHO5.kichthuoc .0086363 1 .0086363 .124 .7295
RESIDUAL 3.5399402 51 .0694106
---------------------------------------------------------------------------
-----
TOTAL (CORRECTED) 3.5487134 53
---------------------------------------------------------------------------
-----
0 missing values have been excluded.
All F-ratios are based on the residual mean square error.
Table of Least Squares Means for CHO5.A
---------------------------------------------------------------------------
-----
95%
Confidence
Level Count Average Stnd. Error for mean
---------------------------------------------------------------------------
-----
GRAND MEAN 54 .7903141 .0377690 .7144723.8661558
A:CHO5.buongtrung
1 26 .7855320 .0557147 .6736546.8974095
2 28 .7950961 .0498658 .6949635.8952287
B:CHO5.kichthuoc
1 35 .8039689 .0448452 .7139177.8940200
2 19 .7766593 .0619596 .6522418.9010767
---------------------------------------------------------------------------
---
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Analysis of Variance for CHO5.B - Type I Sums of Squares
---------------------------------------------------------------------------
-----
Source of variation Sum of Squares d.f. Mean square F-ratio Sig.
level
---------------------------------------------------------------------------
-----
MAIN EFFECTS
A:CHO5.buongtrung .0001369 1 .0001369 .002 .9652
B:CHO5.kichthuoc .0086363 1 .0086363 .124 .7295
RESIDUAL 3.5399402 51 .0694106
---------------------------------------------------------------------------
-----
TOTAL (CORRECTED) 3.5487134 53
---------------------------------------------------------------------------
-----
0 missing values have been excluded.
All F-ratios are based on the residual mean square error.
Table of Least Squares Means for CHO5.B
---------------------------------------------------------------------------
-----
95%
Confidence
Level Count Average Stnd. Error for mean
---------------------------------------------------------------------------
-----
GRAND MEAN 54 .2096859 .0377690 .1338442.2855277
A:CHO5.buongtrung
1 26 .2144680 .0557147 .1025905.3263454
2 28 .2049039 .0498658 .1047713.3050365
B:CHO5.kichthuoc
1 35 .1960311 .0448452 .1059800.2860823
2 19 .2233407 .0619596 .0989233.3477582
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---
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