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Enzymes at work
Rethink Tomorrow...
Contents
1. Why use enzymes for industrial processes? 6
. the nature of enzymes 9 2.1 Chemical reactions under mild conditions 9
2.2 Highly specific action 9
5.2.2 Liming 19 5.2.3 Bating 19 5.2.4 Acid bating 19 5.2.5 Degreasing/fat dispersion 19
5.2.6 Area expansion 20
2.3 Very high reaction rates 9 5. forest products 0
2.4 Numerous enzymes for different tasks 9
. industrial enzyme production 10
. enzymes for detergents and personal care 1
4.1 Laundry detergents and automatic
5.3.1 Traditional pulp and paper processing 20 5.3.2 Amylases for starch modification for paper coatings 21 5.3.3 Xylanases for bleach boosting 21 5.3.4 Lipases for pitch control 21 5.3.5 Esterases for stickies control 21
5.3.6 Enzymes for deinking 21
dishwashing detergents 12 5. animal feed
4.1.2 The role of detergent enzymes
4.1.3 Enzymes for cleaning-in-place (CIP)
13 5.4.1 The use of phytases 23
5.4.2 NSP-degrading enzymes 23
and membrane cleaning in the food industry 13 4.2 Personal care 13
5. enzyme applications in nonfood industries 1
5.5 oil and gas drilling 5.6 Biopolymers 5.7 fuel ethanol 5
5.8 enzymes in organic synthesis – Biocatalysis 6
5.1 textiles 15 5.8.1 Enzymes commonly used for organic synthesis 26
5.1.1 Enzymatic desizing of cotton fabric
5.1.2 Enzymes for denim finishing
15 5.8.2 Enantiomerically pure compounds 28
16
5.1.3 Cellulases for the BioPolishing of cotton fabric
5.1.4 Cellulases for the BioPolishing of lyocell
17 6. enzyme applications in the food industry 9
17 6.1 sweetener production 9
5.1.5 Enzymes for wool and silk finishing
5.1.6 Scouring with enzymes
17 6.1.1 Enzymes for starch modification 30
18 6.1.2 Tailor-made glucose syrups 30
5. leather 18 6.1.3 Processing and enzymology 30 5.2.1 Soaking 18 6.1.4 Sugar processing 32
6. Baking 6.7.4 Fruit preparations 45
6.2.1 Flour supplementation
6.2.2 Dough conditioning
34 6.7.5 Winemaking 45
35 6.7.6 Oil extraction 46
6.2.3 The synergistic effects of enzymes 36 6.8 enzymatic modification of lipids 6 6.2.4 Reduction of acrylamide content in food products 36 6.8.1 Enzymatic degumming 46 6. dairy products 6 6.8.2 Enzymes in simple fat production 47
6.3.1 Cheesemaking
6.3.2 Rennet and rennet substitutes 6.3.3 Cheese ripening
6.3.4 Infant milk formulas
36 6.9 Reduction of viscosity in general 47 37
37 7. safety 9
38
6. Brewing 8 8. enzyme regulation and quality assurance 50
6.4.1 Mashing
6.4.2 Brewing with barley
6.4.3 General filtration problems
6.4.4 Enzymes for improving fermentation
6.4.5 Diacetyl control
38 8.1 Detergent enzymes 50 39 8.2 Food enzymes 50 39
39 9. enzyme origin and function 51
40 9.1 Biochemical synthesis of enzymes 51
6.5 distilling – potable alcohol 0 9.2 How enzymes function 51
6.5.1 Starch liquefaction 6.5.2 Starch saccharification
6.5.3 Viscosity reduction – High gravity fermentation
41 9.3 Basic enzyme kinetics 54 41
41 10. a short history of industrial enzymes 56
6.6 protein hydrolysis for food processing 1
6.6.1 Flavor enhancers 6.6.2 Meat extracts
6.6.3 Pet food
42 11. production microorganisms 58 42
43 1. future prospects – in conclusion 59
6.7 extraction of plant material
6.7.1 Plant cell walls and specific enzyme activities 6.7.2 Fruit juice processing
6.7.3 Citrus fruit
43 1. glossary 60 44
44 1. literature 6
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