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PHỤ LỤC
Phụ lục I: Thành phần các hóa chất trong phản ứng PCR
TAE buffer 50X:
+Tris-HCl 242 g/l.
+Glacial acetic acid 57,1 ml/l.
+EDTA 0,5M, pH 8 100 ml/l.
TE
+Tris HCl 10 mM, pH 8,3.
+EDTA 1mM.
Loading buffer
+100 % glycerol 5 ml.
+TE 5X buffer 2,5 ml.
+Bromphenol Blue 0,015 ml.
Ethidium bromide
+15 l Ethidiumbromide.
+300 ml TAE 0,5 X.
Ladder
+65 % TAE 0,5X.
+25 % loading dye 6X.
+15 % ladder.
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Phụ lục II: Kết quả đọc trình tự với primer ITS4
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Phụ lục III: Kết quả đọc trình tự PUL-Q2-4 với primer ITS5
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Phụ lục IV: Kết quả đọc trình tự RN-LA-10 với primer ITS4
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Phụ lục V: Kết quả đọc trình tự RN-LA-10 với primer ITS5
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