Tài liệu miễn phí Sinh học

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DNA methylation and miRNA-1296 act in concert to mediate spatiotemporal expression of KPNA7 during bovine oocyte and early embryonic development

Epigenetic regulation of oocyte-specific maternal factors is essential for oocyte and early embryonic development. KPNA7 is an oocyte-specific maternal factor, which controls transportation of nuclear proteins important for early embryonic development.

12/29/2020 5:34:22 PM +00:00

Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary

During Drosophila oogenesis, the follicular epithelium differentiates into several morphologically distinct follicle-cell populations. Characteristic bioelectrical properties make this tissue a suitable model system for studying connections between electrochemical signals and the organisation of the cytoskeleton.

12/29/2020 5:34:11 PM +00:00

Survival of Polyploid hybrid salamander embryos

Animals with polyploid, hybrid nuclei offer a challenge for models of gene expression and regulation during embryogenesis. To understand how such organisms proceed through development, we examined the timing and prevalence of mortality among embryos of unisexual salamanders in the genus Ambystoma.

12/29/2020 5:34:05 PM +00:00

YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation

Immature mammalian oocytes are held arrested at prophase I of meiosis by an inhibitory phosphorylation of cyclin-dependent kinase 1 (CDK1). Release from this meiotic arrest and germinal vesicle breakdown is dependent on dephosphorylation of CDK1 by the protein, cell cycle division 25B (CDC25B).

12/29/2020 5:33:54 PM +00:00

A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice

Mouse NOTCH1 carries a highly conserved O-fucose glycan at Thr466 in epidermal growth factor-like repeat 12 (EGF12) of the extracellular domain. O-Fucose at this site has been shown by X-ray crystallography to be recognized by both DLL4 and JAG1 Notch ligands.

12/29/2020 5:33:33 PM +00:00

Sonic hedgehog promotes chondrogenesis of rabbit bone marrow stem cells in a rotary cell culture system

Sonic hedgehog (Shh) is an important signalling protein involved in the induction of early cartilaginous differentiation. Herein, we demonstrate that Shh markedly induces chondrogenesis of rabbit bone marrow stromal cells (BMSCs) under microgravity conditions, and promotes cartilage regeneration.

12/29/2020 5:33:24 PM +00:00

Role of PRY-1/Axin in heterochronic miRNAmediated seam cell development

Caenorhabditis elegans seam cells serve as a good model to understand how genes and signaling pathways interact to control asymmetric cell fates. The stage-specific pattern of seam cell division is coordinated by a genetic network that includes WNT asymmetry pathway components WRM-1, LIT-1, and POP-1, as well as heterochronic microRNAs (miRNAs) and their downstream targets.

12/29/2020 5:33:16 PM +00:00

Deletion of Tet proteins results in quantitative disparities during ESC differentiation partially attributable to alterations in gene expression

The Tet protein family (Tet1, Tet2, and Tet3) regulate DNA methylation through conversion of 5- methylcytosine to 5-hydroxymethylcytosine which can ultimately result in DNA demethylation and play a critical role during early mammalian development and pluripotency.

12/29/2020 5:33:09 PM +00:00

Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst

Prostate androgen-regulated mucin-like protein 1 (PARM1) is a pro-proliferative and anti-apoptotic glycoprotein involved in the endoplasmic reticulum (ER) stress response. A single nucleotide polymorphism in the coding region of PARM1 has been associated with competence of bovine embryos to develop to the blastocyst stage.

12/29/2020 5:33:00 PM +00:00

Insulin signaling mediates previtellogenic development and enhances juvenile hormone-mediated vitellogenesis in a lepidopteran insect, Maruca vitrata

Insulin/insulin-like growth peptide signaling (IIS) down-regulates hemolymph sugar level and facilitates larval growth in the soybean pod borer, Maruca vitrata. The objective of this study is to determine whether IIS of M. vitrata can mediate ovarian development of adult females.

12/29/2020 5:32:49 PM +00:00

A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos

The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence shows that MEK signalling plays a limited role in the segregation of this lineage.

12/29/2020 5:32:40 PM +00:00

Electrochemical patterns during Drosophila oogenesis: Ion-transport mechanisms generate stage-specific gradients of pH and membrane potential in the follicle-cell epithelium

Alterations of bioelectrical properties of cells and tissues are known to function as wide-ranging signals during development, regeneration and wound-healing in several species. The Drosophila follicle-cell epithelium provides an appropriate model system for studying the potential role of electrochemical signals, like intracellular pH (pHi ) and membrane potential (Vmem), during development.

12/29/2020 5:32:30 PM +00:00

Growth and cellular patterning during fetal human inner ear development studied by a correlative imaging approach

Progressive transformation of the otic placode into the functional inner ear during gestational development in humans leads to the acquisition of hearing perception via the cochlea and balance and spatial orientation via the vestibular organ.

12/29/2020 5:32:20 PM +00:00

An annual cycle of gene regulation in the red-legged salamander mental gland: From hypertrophy to expression of rapidly evolving pheromones

Cell differentiation is mediated by synchronized waves of coordinated expression for hundreds to thousands of genes, and must be regulated to produce complex tissues and phenotypes. For many animal species, sexual selection has driven the development of elaborate male ornaments, requiring sex-specific differentiation pathways.

12/29/2020 5:32:07 PM +00:00

Androgen receptor expression is required to ensure development of adult Leydig cells and to prevent development of steroidogenic cells with adrenal characteristics in the mouse testis

The interstitium of the mouse testis contains Leydig cells and a small number of steroidogenic cells with adrenal characteristics which may be derived from the fetal adrenal during development or may be a normal subset of the developing fetal Leydig cells.

12/29/2020 5:31:53 PM +00:00

Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish

C-lectin family 14 Member A (Clec14a) is a transmembrane protein specifically expressed in vascular endothelial cells during embryogenesis. Previous in vitro and in vivo studies have provided conflicting data regarding Clec14a role in promoting or inhibiting angiogenesis, therefore its functional role in vascular development remains poorly understood.

12/29/2020 5:31:40 PM +00:00

Molecular cloning and characteristics of DnaJa1and DnaJb1 in Coilia nasus: Possible function involved in oogenesis during spawning migration

Coilia nasus oogenesis/spawning migration is a well-defined synchronous arrangement process. DnaJs are indispensable molecular chaperones for oogenesis process. However, how DnaJs involved the anadromous spawning migration mechanism is outstanding and plausible.

12/29/2020 5:31:26 PM +00:00

Differential roles of insulin like growth factor 1 receptor and insulin receptor during embryonic heart development

The embryonic day E10–13 period of mouse heart development is characterized by robust cardiomyocyte proliferation that creates the compact zone of thickened ventricular wall myocardium. This process is initiated by the formation of the epicardium on the outer heart surface, which releases insulinlike growth factor 2 (IGF2) as the primary cardiomyocyte mitogen.

12/29/2020 5:31:17 PM +00:00

Expression of NK genes that are not part of the NK cluster in the onychophoran Euperipatoides rowelli (Peripatopsidae)

NK genes are a group of homeobox transcription factors that are involved in various molecular pathways across bilaterians. They are typically divided into two subgroups, the NK cluster (NKC) and NK-linked genes (NKL).

12/29/2020 5:30:44 PM +00:00

Vitellogenins in the spider Parasteatoda tepidariorum – expression profile and putative hormonal regulation of vitellogenesis

Knowledge about vitellogenesis in spiders is rudimentary. Therefore, the aim of study was to check the vitellogenin (Vg) presence in various tissues of the female spider Parasteatoda tepidariorum, determine when and where vitellogenesis starts and takes place, and the putative role of selected hormones in the vitellogenesis.

12/29/2020 5:30:36 PM +00:00

Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms

Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used spinner flask and its correlation with the successful generation of brain organoids has not been determined.

12/29/2020 5:30:29 PM +00:00

Interleukin-6 increases inner cell mass numbers in bovine embryos

Work in other species suggests that interleukin-6 (IL6) promotes early embryo development. It was unclear whether IL6 serves as an embryokine in cultured bovine embryos. This work was undertaken to elucidate the role of IL6 during in vitro bovine embryo production.

12/29/2020 5:30:23 PM +00:00

Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components

BMP signaling is involved in myriad metazoan developmental processes, and study of this pathway in Drosophila has contributed greatly to our understanding of its molecular and genetic mechanisms. These studies have benefited not only from Drosophila’s advanced genetic tools, but from complimentary in vitro culture systems.

12/29/2020 5:30:15 PM +00:00

Environmental cycles regulate development time via circadian clock mediated gating of adult emergence

Previous studies have implicated a role for circadian clocks in regulating pre-adult development of organisms. Among them two approaches are most notable: 1) use of insects whose clocks have different freerunning periods and 2) imposition of artificial selection on either rate of development, timing of emergence or circadian period in laboratory populations.

12/29/2020 5:30:09 PM +00:00

Histone acetyltransferase CBP-related H3K23 acetylation contributes to courtship learning in Drosophila

Histone modifications are critical in regulating neuronal processes. However, the impacts of individual histone modifications on learning and memory are elusive. Here, we investigated the contributions of histone H3 lysine modifications to learning and memory in Drosophila by using histone lysine-to-alanine mutants.

12/29/2020 5:30:02 PM +00:00

Fermitin family homolog-2 (FERMT2) is highly expressed in human placental villi and modulates trophoblast invasion

Integrins are transmembrane receptors that mediate cell–extracellular matrix (ECM) and cell-cell adhesion and trophoblast cells undergo changes in integrin expression as they differentiate. However, the mechanism(s) of integrin activation leading to integrin-mediated signaling in trophoblast cell differentiation is unknown.

12/29/2020 5:29:49 PM +00:00

Effects of sex on response of the bovine preimplantation embryo to insulin-like growth factor 1, activin A, and WNT7A

Alterations in maternal environment can sometimes affect embryonic development in a sexuallydimorphic manner. The objective was to determine whether preimplantation bovine embryos respond to three maternally-derived cell signaling molecules in a sex-dependent manner.

12/29/2020 5:29:43 PM +00:00

Caspases and matrix metalloproteases facilitate collective behavior of non-neural ectoderm after hindbrain neuropore closure

Mammalian brain is formed through neural tube closure (NTC), wherein both ridges of opposing neural folds are fused in the midline and remodeled in the roof plate of the neural tube and overlying non-neural ectodermal layer. Apoptosis is widely observed from the beginning of NTC at the neural ridges and is crucial for the proper progression of NTC, but its role after the closure remains less clear.

12/29/2020 5:29:33 PM +00:00

Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach

Dmrt2a is a zinc finger like transcription factor with several roles during zebrafish early development: left-right asymmetry, synchronisation of the somite clock genes and fast muscle differentiation. Despite the described functions, Dmrt2a mechanism of action is unknown.

12/29/2020 5:29:25 PM +00:00

Swimming exercise to control precocious maturation in male seabass (Dicentrarchus labrax)

Male European seabass, already predominant (~ 70%) in cultured stocks, show a high incidence (20–30%) of precocious sexual maturation under current aquaculture practices, leading to important economic losses for the industry.

12/29/2020 5:29:18 PM +00:00