Tài liệu miễn phí Sinh học

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Safety and cross protective efficacy of bacteriophage lysate against Pasteurella multocida infection in mice

In this study established the methodology to isolate highly virulent bacteriophage and assessed the ability of this phage lysate to induce protection against wild-type challenge in mice and the natural host chickens. Subcutaneous administration of phage lysate to mice stimulated significant protection against Pasteurella multocida P52 and A1 serotypes. But, significantly phage lysate was able to induce protection through subcutaneous route against challenge with wild type Pasteurella multocida A1 serotype in chicken but not through intranasal administration of phage lysates

5/19/2021 11:41:14 PM +00:00

Microbial profile of a calcareous soil of south Tamil Nadu, India

Indian farmers are highly dependent on rainfall and all activities of agriculture starting from sowing to post harvest are affected either directly or indirectly by weather especially by rainfall. In the vagaries of weather condition and make it us suitable condition for cultivation to utilize such type of soil for improving the agricultural activity without any yield reduction.

5/19/2021 11:38:42 PM +00:00

Biosensors and bioremediation as biotechnological tools for environmental monitoring and protection

Their use in fields of environmental pollution control and early warning is still in the early stages. Biosensors are classified according to their transduction principle such as optical, electrochemical and piezoelectric or based on their recognition element as immunosensors, apt sensors, genosensors, and enzymatic biosensors, when antibodies, aptamers, nucleic acids, and enzymes are, respectively, used.

5/19/2021 11:25:21 PM +00:00

Microbial diversity in the rhizosphere of some wild plants in Riyadh region, Saudi Arabia

The present study had been carry out on selected local plants that usually found as wild flora in some meadows of Riyadh region. Diversity and densities of different microorganisms present in the rhizosphere of selected wild plants growing in these meadows were determined.

5/19/2021 11:23:18 PM +00:00

Bacterial bioremediation of imidacloprid in mango orchard soil by Pseudomonas mosselii strain NG1

The present study was undertaken to isolate different microbes from imidacloprid treated mango orchard soil and apply the most effective one to study its ability to degrade imidacloprid in soil under field condition.

5/19/2021 11:15:14 PM +00:00

Occurrence and abundance of cyanobacterial strains and physico-chemical properties of water bodies

The vulnerability of cyanobacteria to environmental impact and their possible depletion or extinction like other microalgae necessitated the understanding of the distribution of cyanobacterial population in selected aquatic bodies which are important to economic, recreational and religious activity of people. It was also considered important to characterize cyanobacterial population in relation to physico-chemical parameters of water bodies.

5/19/2021 11:14:23 PM +00:00

Towards the development of protein expression by inducible ecdysone system

The ecdysone inducible system was generated for checking the toxicity of target gene which was transected into the cells. The transcription unit, modified ecdysone receptor (IND) was cloned into vector pcDNA-Dest40 to form the new inducible expression plasmid pDest40-IND via cloning technique. A clear band with size about 487bp of PCR product indicated the success of amplification IND promoter.

5/19/2021 11:05:45 PM +00:00

A study on multiplication of oncidium-sweet sugar by using cell tissue culture

This study is to research on a process of rapid multiplication of Oncidium-Sweet Sugar Orchids by using cell tissue culture in order to create high quality varieties that help serve flower production industry. This is also hoped to contribute the Orchids production in Thanh Hoa province.

5/19/2021 11:05:27 PM +00:00

Generation stable cell line used for protein expression system

The ecdysone inducible system was generated for checking the the toxicity of the target gene which was transfected into the cells. The retinoid X receptor (RXR) was transfected into HEK293FT to generate an inducible stable cell line pVgRXR-HEK293FT. For checking the function, the cells transfected with pIND-GFP and induced by 1µM ponasteron A for GFP expression. The fluorescence signals were detected in the transfected and induced cells informed the success of experiment.

5/19/2021 11:03:43 PM +00:00

Nursery conditions for Oncidium (sweet sugar) growth and development at seedling and adult stages

Production of Orchids in Viet Nam has not fully developed, compared with the potentials of this kind of flowers due to a lack of appropriate process of technology to produce Orchids. Research on techniques to grow Oncidium-Sweet Sugar Orchids in the period of seedling and production with a hope of contributing to the development of Orchids in Viet Nam.

5/19/2021 11:03:13 PM +00:00

Rapid in vitro propagation of native Dendrobium chrysotoxum

The basic MS containing 20g/l sucrose, 8g/l agar, TDZ 4μM and NAA 1μM was the most optimum medium for shoot propagation from seedlings and gave the best shoot quality. NAA had little effect on generating roots of Dendrobium chrysotoxum in vitro.

5/19/2021 11:02:14 PM +00:00

A trial of Neolamarckia cadamba in Thanh Hoa province

The representative of the ecological regions in Thanh Hoa province chosen for the study included: Thuong Xuan district (a representive of the mountainous area), Nhu Thanh district (a representive of the midland area), and Tinh Gia district (a representive of delta coastal area).

5/19/2021 11:00:32 PM +00:00

Nghiên cứu nhân giống hoa Lan vũ nữ (Oncidium) bằng phương pháp nuôi cấy mô tế bào

Hoa lan là một loài hoa quí, mang lại giá trị kinh tế rất cao trong ngành sản xuất kinh doanh hoa. Tuy nhiên, việc sản xuất hoa lan trong đó có lan Vũ nữ (Oncidium), nguồn cung cấp giống ở Việt Nam vẫn còn nhiều hạn chế. Bài báo đưa ra qui trình nhân nhanh giống lan Vũ nữ (Oncidium), tạo ra cây giống có chất lượng cao cung cấp cho sản xuất với quy mô công nghiệp.

5/19/2021 10:48:15 PM +00:00

Thành phần sâu, nhện hại nhãn, đặc điểm hình thái, sinh học và diễn biến mật độ của rầy vân nâu Cornegenapsilla sinica (Homoptera: Psyllidae) trên nhãn năm 2006, tại thị xã Hưng Yên

Bài ciết này đề cập đến thành phần sâu, nhện hại nhãn; đặc điểm hình thái, sinh học và diễn biến mật độ của Rầy vân nâu Cornegenapsilla sinica, tại thị xã Hưng Yên trong vụ nhãn 2006.

3/19/2021 1:58:20 AM +00:00

Chu kỳ sinh học và ứng dụng

Người ta thường cho rằng mỗi người có một số phận. Có nhiều cách kiếm giải khác nhau, tuy nhiên, một trong những nghiên cứu khách quan, khoa học đã cho thấy mỗi người đều có những nhịp sinh học riêng ảnh hưởng trực tiếp đến đời sống, sức khỏe và hạnh phúc của bản thân. Bài viết nêu một số kiến thức cơ bản về chu kỳ sinh học (nhịp sinh học) và ứng dụng trong cuộc sống.

3/18/2021 7:25:05 PM +00:00

Tối ưu hóa quá trình chiết xuất pectin từ vỏ chuối và ứng dụng tạo màng bao bảo quản mận

Bài viết tiến hành tối ưu hóa một số điều kiện ảnh hưởng đến hàm lượng pectin chiết tách từ vỏ chuối như nhiệt độ, thời gian, nồng độ axit citric bằng cách thiết kế thí nghiệm và tính toán trên phần mềm Design Explorer (phiên bản 7.1 Trial, Stat-EaseInc., Minneapolis, USA).

3/18/2021 5:53:01 PM +00:00

Tính toán thời gian cấp đông thực phẩm dạng trụ vô hạn và cầu

Mục tiêu nghiên cứu của bài viết này là đề xuất phương pháp dự đoán thời gian đóng băng cho thực phẩm có hình dạng trụ vô hạn và cầu mà không dùng hệ số quy đổi.

3/18/2021 5:51:36 PM +00:00

Studies on biology of greater wax moth (Galleria mellonella L.)

The information on the biology parameters of greater wax moth is little scanty. Hence, the present study was conducted to know the biological parameters of greater waxmoth.

3/18/2021 5:11:16 PM +00:00

Evaluation of different phenotypic methods versus polymerase chain reaction for detection of plasmid mediated AmpC β-lactamase-producing strains of Proteus mirabilis

The aim of this study is to evaluate and comparing routinely phenotypic methods in detection of resistance with molecular methods. From this study, it can be concluded that cephamycin-Hodge test is the most sensitive, specific, interpretable and efficient test for detection of AmpC β-lactamases in clinical isolates of Proteus mirabilis, compared to the molecular method.

3/18/2021 5:08:29 PM +00:00

Effect of physico-chemical conditions on the structure and composition of the phytoplankton community at Jatinangor, Indonesia

This study was conducted to evaluate the impact of activities in campus Universitas Padjadjaran (UNPAD) on the water quality as a result of the entery and discharge of pollution materials from laboratories, food courts, agriculture and domestic waste in campus UNPAD to Check dam UNPAD, Ciparanje and Cikuda River. Very limited information is available on the phytoplankton status and the effect of water pollution on the phytoplankton population of this area.

3/18/2021 5:07:51 PM +00:00

Effects of electron beam irradiation on microbial quality of pork sausage stored at refrigeration temperature

This view of limited information on electron beam irradiated meat products, the scientific study is therefore undertaken to assess its microbiological, physico-chemical and sensory evaluation of electron beam irradiated meat products in order to determine the shelflife henceforth provide safe products to consumer.

3/18/2021 5:05:58 PM +00:00

MicroRNAs as early detection biomarker in cancer

The expression profiling of miRNAs has clearly assessed their role as diagnostic and prognostic biomarkers which will help clinicians to assess tumor initiation, progression and response to treatment in cancer patients. In the development of various stages of cancer, the role and involvement of miRNAs has been revealed. This review focuses on the association of miRNAs with that of cancer and highlights the role of miRNAs in clinical practice, such as diagnosis, prognosis and detection.

3/18/2021 5:05:45 PM +00:00

Prevalence of multidrug resistant uropathogenic E. coli: Role of β-lactamase

The study was carried out from January 2017 to June 2017 at the Microbiology Diagnostics and Infection Control Unit at the Medical Microbiology and immunology Department, Faculty of Medicine, Mansoura University.

3/18/2021 5:05:13 PM +00:00

An investigation on the heavy metal tolerance and antibiotic profile of the Pantoea agglomerans UCP1320

The aim of this study is to determine the antimicrobial resistance profile patterns to 15 antibiotics and heavy metals (Zn+2, Cu+2 and Cd+2) by Pantoeaagglomerans bacteria. The (MIC) of the heavy metals was varied from 200 µg /mL to 2200 µg/mL. The results showed that the bacteria were resistant to Zn+2, Cu+2 and Cd+2, considering the MIC values compared with the strain Escherichia coli K-12 used as control. P. agglomeransshowed an antibiotic profile of resistance to Cefepime, Cefotaxime, Cefpodoxime, Clindamycin, and Amikacin, and sensitivity to Penicillin, and other antibiotics, thus suggesting that genetically-determined systems for resistance to toxic heavy metals was observed.

3/18/2021 5:03:20 PM +00:00

Isolation, identification, characterization and enzymatic profile of the new strain of Pantoea agglomerans

The objective of this work was to identify and characterize biochemical, morphological and enzymatic activity, as well as the influence of abiotic factors in a Pantoea isolated from industrial laundry effluent in Pernambuco. The results showed that the bacterium presents characteristics similar to those presented by the species of Pantoea agglomerans; however, the biochemical and morphological tests were not enough to accurately identify the species.

3/18/2021 5:03:14 PM +00:00

Use of kinetic and thermodynamic parameters for the prevention of enzymatic browning of edible yam Dioscorea cayenensis-rotundata cv. “Zrèzrou”

The effect of heat treatment on edible yam (Dioscorea cayenensis-rotundata cv. “Zrèzrou”) polyphenol oxidase activity was studied over a range of 35 to 75°C. Under all conditions investigated, a first-order kinetic model could describe the thermal inactivation data with k-values between 0.004 and 0.108 min-1 .

3/18/2021 5:01:44 PM +00:00

Characterization of microbial lipid production with mucor rouxii on pure carbon substrates

In this chapter, physiological responses of oleaginous fungi such as, biomass production, substrate uptake, secretion of hydrolytic enzymes, and lipid accumulation with cellulose or starch as the organic carbon substrate were studied. Glucose, one of the simplest sugars, was also studied as a comparison study. M. rouxii, a known lipid and GLA producer (Ahmed et al., 2006), was used in this study.

3/18/2021 5:01:32 PM +00:00

Effects of antioxidants in controlling phenolic exudates in in vitro culture of Gliricidia [Gliricidia sepium (Jacq.) Steud.]

The present investigation was carried out to study the influence of antioxidants on in vitro culture of Gliricidia sepium. Soft nodal stem segments were inoculated on MS medium supplemented with desired concentrations plant growth regulator such as cytokinins (BAP/Kinetin) and different antioxidants. Antioxidants viz., activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation.

3/18/2021 4:59:17 PM +00:00

Optimization of arginine deaminase production from indigenous bacterium pseudomonas aeruginosa PS2

In the present investigation, we have described optimization of environmental and nutritional requirements for maximum biosynthesis of ADI from bacterium Pseudomonas aeruginosa PS2. We observed that batch time 25 h, inoculum age 20 h, 8% (v/v) inoculum size, pH 6.5 and temperature 37 °C were found as the most suitable operating conditions for ADI production. Galactose, peptone, KH2PO4 and L-arginine were found as the best carbon, nitrogen, mineral ion and inducer for ADI production, respectively.

3/18/2021 4:59:10 PM +00:00

Effect of non-polar bioactive molecules of tagetus on second stage larvae of meloidogyne javanica in lab conditions

The paper deals with the study of the effect of three different, non-polar compounds fractionated at different levels of polarity and their impact on juveniles.

3/18/2021 4:58:45 PM +00:00