The biological changes underlying the transition process from gingival health to early inflammatory changes involve local increase in vascular permeability, edema and the recruitment and activation of polymorphonuclear neutrophils (PMN) (Delima and Van Dyke 2003). Acquired immune response becomes involved once antigen-presenting cells interact with immunocompetent cells, such as T and B lymphocytes, leading to the expansion of antibody-secreting plasma cells and the development of the chronic le