Micromanipulation involves a well-integrated set of technologies in assisted reproductive technology (ART). Its applications are diagnostic as well as therapeutic, and it is practiced in mature gametes and all stages of preimplantation embryos. It is used in biopsy for preimplantation genetic diagnosis (PGD), intra-cytoplasmic sperm injection (ICSI), assisted hatching, and in other more controversial areas such as egg freezing via zygote reconstitution, cryopreservation of isolated testicular sp