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David Bakewell Micro- and Nano-Transport of Biomolecules Download free ebooks at bookboon.com 2 Micro- and Nano- Transport of Biomolecules © 2009 David Bakewell & Ventus Publishing ApS ISBN 978-87-7681-513-4 Download free ebooks at bookboon.com 3 Micro- and Nano-Transport of Biomolecules Contents Contents Preface 7 1 Introduction 8 1.1 Motivation: biomolecules in scientifi c context 8 1.2 Length scale of transport 10 1.3 Biomolecule transport example: engineered microdevices 11 1.4 Structure of this e-book 13 2 Biomolecules and their electrical properties 14 2.1 Biomolecules in cells 14 2.2 Biomolecules: structure and function 16 2.2.1 Nucleic acids 16 2.2.2 Proteins 20 2.2.3 Carbohydrates 21 2.2.4 Lipids 22 2.3 Biomolecules: electrical properties 22 2.3.1 Polyelectrolytes 22 2.3.2 DNA can be modeled as wormlike chain 24 2.3.3 Biomolecules and bioparticles 25 2.3.4 Electrical double layer 25 2.3.5 Introduction to dielectric polarization 27 2.3.6 Polarisation parameters: a brief view 28 2.3.7 Measurement of biomolecule polarisation parameters 29 2.4 Concluding remarks 30 The next step for top-performing graduates Masters in Management Designed for high-achieving graduates across all disciplines, London Business School’s Masters in Management provides specific and tangible foundations for a successful career in business. This 12-month, full-time programme is a business qualification with impact. In 2010, our MiM employment rate was 95% within 3 months of graduation*; the majority of graduates choosing to work in consulting or financial services. As well as a renowned qualification from a world-class business school, you also gain access to the School’s network of more than 34,000 global alumni – a community that offers support and opportunities throughout your career. For more information visit www.london.edu/mm, email mim@london.edu or give us a call on +44 (0)20 7000 7573. * Figures taken from London Business School’s Masters in Management 2010 employment report Download free ebooks at bookboon.com 4 Micro- and Nano-Transport of Biomolecules Contents 3 Moving biomolecules using electric fi elds 31 3.1 Electrophoresis 31 3.2 Dielectrophoresis (DEP) 32 3.2.1 Polarisation and DEP biomolecule transport 36 3.2.2 Maxwell-Wagner interfacial polarisation 37 3.2.3 Maxwell-Wagner interfacial polarisation for bioparticles 38 3.2.4 Maxwell-Wagner polarisation for DNA 41 3.2.5 Counterion fl uctuation polarisation 43 3.2.6 Counterion fluctuation polarisation for bioparticles 43 3.2.7 Counterion fluctuation polarisation for DNA 46 3.2.8 Other polarisation mechanisms 51 3.3 Micro-environments for biomolecule transport 51 3.4 Concluding remarks 52 4 Basic micro- and nano-transport 54 4.1 Inertial, friction and sedimentation forces on single biomolecules 54 4.2 Electromagnetic forces acting on single biomolecules 56 4.2.1 Electric fields and electrophoresis 56 4.2.2 Inhomogenous electric fields and dielectrophoresis 57 4.2.3 Electroosmosis 60 4.2.4 Magnetic fi elds 60 4.3 Thermal fl uctuations 61 4.4 Combining forces for predicting single bioparticle trajectory 64 4.5 Langevin equation for a single bioparticle (biomolecule) 64 4.6 Langevin equation stochastic integration and the modified diffusion equation (MDE) 65 4.6.1 Example of one-dimensional (1D) MDE transport 67 Teach with the Best. Learn with the Best. Agilent offers a wide variety of affordable, industry-leading electronic test equipment as well as knowledge-rich, on-line resources —for professors and students. We have 100’s of comprehensive web-based teaching tools, lab experiments, application notes, brochures, DVDs/ CDs, posters, and more. © Agilent Technologies, Inc. 2012 See what Agilent can do for you. www.agilent.com/find/EDUstudents www.agilent.com/find/EDUeducators u.s. 1-800-829-4444 canada: 1-877-894-4414 Download free ebooks at bookboon.com 5 ... - tailieumienphi.vn
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