Methods in Modern Biophysics - Second Edition

Methods in Modern Biophysics Bengt Nölting Methodsin ModernBiophysics Second Edition With 267 Figures 123 Dr. Bengt Nölting Prussian Private Institute of Technology at Berlin Am Schlosspark 30 D-13187 Berlin Germany nolting@pitb.de Library of Congress Control Number: 2005929410 ISBN-10 3-540-27703-X 2nd Edition Springer Berlin Heidelberg New York ISBN-13 978-3-540-27703-3 2nd Edition Springer Berlin Heidelberg New York 2nd edition 2006. ISBN 3-540-01297-4 1st Edition Springer Berlin Heidelberg New York This work is subject to copyright. All rights reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks.Duplicationofthispublicationorpartsthereofispermittedonlyundertheprovisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable for prosecution under the German Copyright Law. Springer is a part of Springer Science+Business Media springeronline.com © Springer-Verlag Berlin Heidelberg 2003, 2006 Printed in Germany The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publisher cannot guarantee the accuracy of any information about dosage and application contained in this book. In every individual case the user must check such information by consulting the relevant literature. Patents:Anumberofmethodsmentionedinthisbookarecoveredbypatents.Nothinginthis publication should be construed as an authorization or implicit license to practice methods covered by any patents. The instructions given forcarryingoutpractical experiments do not absolve the reader from being responsible for safety precautions. Liability is not accepted by the authors. Safety considerations: Anyone carrying out these methods will encounter pathogenic and infectiousbiologicalagents,toxicchemicals,radioactivesubstances,highvoltageandintense lightradiationwhicharehazardousorpotentiallyhazardousmaterialsormatter.Itisrequired that these materials and matter be used in strict accordance with all local and national regulations and laws. Users must proceed with the prudence and precaution associated with good laboratory practice, under the supervision of personnel responsible for implementing laboratory safety programs at their institutions. Typesetting: By the Author Production: LE-T X, Jelonek, Schmidt & Vöckler GbR, Leipzig Coverdesign: KuenkelLopka, Heidelberg Printed on acid-free paper 2/YL – 5 4 3 2 1 0 To Alan R. Fersht Robert Huber Manfred Eigen Kurt Wüthrich ... - tailieumienphi.vn